阿扎胞苷对SV40感染的BSC-1细胞的作用。

Effect of azacytidine in SV40-infected BSC-1 cells.

作者信息

Reuveni Y, Rosenthal L J

出版信息

Intervirology. 1979;11(3):191-5. doi: 10.1159/000149033.

Abstract

5-Azacytidine (5-AzaCR) reduced significantly the levels of ribosomal 28S and 18S RNAs in the cytoplasm of uninfected and simian virus 40 (SV40)-infected BSC-1 cells. The percent inhibition of ribosomal RNAs was correlated to both the dose of AzaCR and the time of exposure. In SV40-infected cultures treated with 200 microgram/ml AzaCR, a portion of the total cytoplasmic radioactivity was observed to sediment as 19S and 16S by sucrose gradient analysis. The 19S and 16S peaks represented in part the viral-specific RNA species. This result was due to the drastic reduction (70--75%) of ribosomal RNAs in the cytoplasm of infected cultures.

摘要

5-氮杂胞苷（5-AzaCR）显著降低了未感染和感染猿猴病毒40（SV40）的BSC-1细胞胞质中核糖体28S和18S RNA的水平。核糖体RNA的抑制百分比与AzaCR的剂量和暴露时间均相关。在用200微克/毫升AzaCR处理的SV40感染培养物中，通过蔗糖梯度分析观察到一部分总胞质放射性以19S和16S形式沉降。19S和16S峰部分代表病毒特异性RNA种类。这一结果是由于感染培养物胞质中核糖体RNA急剧减少（70--75%）所致。