Bogers J J, Nibbeling H A, Deelder A M, van Marck E A
Laboratory of Pathology, University of Antwerp (UIA), Belgium.
J Histochem Cytochem. 1996 Jan;44(1):43-8. doi: 10.1177/44.1.8543781.
In this study we compared the recently commercialized electron microscopy embedding resin Unicryl with the well-known resin Lowicryl K4M with regard to morphological and immunohistochemical preservation properties. The standard embedding procedure recommended by the manufacturer for the use of Unicryl resulted in considerable morphological alterations of the tissue, with the appearance of large gaps in and between the cells of the examined tissue. Morphometric analysis pointed to a swelling of the extracellular matrix as the main cause of these morphological artifacts. A slight modification in the protocol to correct this artifact is proposed and tested. Immunohistochemically, tissue embedded in Unicryl resulted in a significantly stronger immunogold labeling than identical tissue embedded in Lowicryl K4M. From the results of this technical study, it can be concluded that Unicryl embedding is a valuable new tool to supplement the available techniques for immunoelectron microscopic studies.
在本研究中，我们将最近商业化的电子显微镜包埋树脂Unicryl与著名的树脂Lowicryl K4M在形态学和免疫组织化学保存特性方面进行了比较。制造商推荐的Unicryl使用标准包埋程序导致组织出现相当大的形态学改变，在所检查组织的细胞内和细胞间出现大的间隙。形态计量分析表明细胞外基质肿胀是这些形态学假象的主要原因。提出并测试了对方案的轻微修改以纠正此假象。免疫组织化学方面，与嵌入Lowicryl K4M的相同组织相比，嵌入Unicryl的组织产生了明显更强的免疫金标记。从这项技术研究的结果可以得出结论，Unicryl包埋是一种有价值的新工具，可补充用于免疫电子显微镜研究的现有技术。
