Slepecky N B, Ogata Y
Institute for Sensory Research, Syracuse University, Syracuse, NY, USA.
Nihon Jibiinkoka Gakkai Kaiho. 1996 Mar;99(3):361-9. doi: 10.3950/jibiinkoka.99.361.
An improved method for embedding specimens in polyethylene glycol (PEG) 4000, a water soluble polymer, was used to prepare the vestibular end-organs of the inner ear. Staining of the tissue sections of PEG embedded specimens with antibodies to alpha-tubulin and to calmodulin was compared with staining of tissue sections of Araldite and Unicryl embedded specimens. PEG embedded sections revealed sensitive immunocytochemical labeling with excellent morphological resolution. The problems of embedding and orienting small specimens of the inner ear in PEG are described and the methods used to solve them are described.
一种改进的将标本嵌入聚乙二醇（PEG）4000（一种水溶性聚合物）的方法被用于制备内耳的前庭终器。将PEG包埋标本的组织切片用抗α-微管蛋白和抗钙调蛋白抗体染色，并与环氧树脂和单组分丙烯酸酯包埋标本的组织切片染色进行比较。PEG包埋切片显示出灵敏的免疫细胞化学标记，且形态分辨率极佳。文中描述了将内耳小标本嵌入PEG时遇到的问题以及解决这些问题所采用的方法。
