Möller G M, de Jong T A, Overbeek S E, van der Kwast T H, Postma D S, Hoogsteden H C
Department of Pulmonary Diseases, Erasmus University, Rotterdam, The Netherlands.
J Histochem Cytochem. 1996 Jan;44(1):67-9. doi: 10.1177/44.1.8543784.
Bronchial biopsies from two patients with atopic asthma were analyzed by immunogold labeling to detect the ultrastructural location of interleukin 5 (IL-5). In eosinophils, IL-5 was localized to the electron-dense crystalloid core compartment of the secondary or specific eosinophil granules. Other structures in the eosinophils were unlabeled. No IL-5 was detected in mast cells. Control sections incubated with an irrelevant primary antibody were negative. This study demonstrates that pre-formed IL-5 is stored within the major population of secondary granules in human eosinophils.
通过免疫金标记分析了两名特应性哮喘患者的支气管活检组织，以检测白细胞介素5（IL-5）的超微结构定位。在嗜酸性粒细胞中，IL-5定位于次级或特异性嗜酸性粒细胞颗粒的电子致密晶体核心区室。嗜酸性粒细胞中的其他结构未被标记。在肥大细胞中未检测到IL-5。用无关一抗孵育的对照切片为阴性。本研究表明，预先形成的IL-5储存在人类嗜酸性粒细胞的主要次级颗粒群体中。
