Quinn B, Graybiel A M
Department of Brain & Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, USA.
J Histochem Cytochem. 1996 Jan;44(1):71-4. doi: 10.1177/44.1.8543785.
Several silver-based procedures have been developed to intensify the peroxidase-catalyzed diaminobenzidine polymer used as a marker in immunohistochemistry. Many of these methods use acutely unstable reagents or reagents that are not amenable to batch processing. We describe a new procedure with an initial treatment in dilute ammoniacal silver, followed by a differentiation step to remove background staining and a final toning in 0.2% gold chloride. The procedure provides a jet-black intensification of the tan diaminobenzidine product, is amenable to batch processing of free-floating sections and slide-mounted sections, and does not require acid-cleaned glassware.
已经开发出几种基于银的方法来增强过氧化物酶催化的二氨基联苯胺聚合物，该聚合物在免疫组织化学中用作标记物。这些方法中的许多使用极不稳定的试剂或不适合批量处理的试剂。我们描述了一种新方法，首先在稀氨银中进行处理，然后进行分化步骤以去除背景染色，最后在0.2%氯化金中进行调色。该方法可使黄褐色的二氨基联苯胺产物强化为亮黑色，适用于对游离切片和载玻片切片进行批量处理，并且不需要酸洗玻璃器皿。
