Gallyas F, Wolff J R
J Histochem Cytochem. 1986 Dec;34(12):1667-72. doi: 10.1177/34.12.3537114.
Physical developers can increase the visibility of end products of certain histochemical reactions, such as oxidative polymerization of diaminobenzidine and selective binding of complex silver iodide ions to Alzheimer's neurofibrillary changes. Unfortunately, this intensification by silver coating is generally superimposed on a nonspecific staining originating from the argyrophil III reaction, which also takes place when tissue sections are treated with physical developers. The present study reveals that the argyrophil III reaction can be suppressed when tissue sections are treated with certain metal ions and hydrogen peroxide before they are transferred to the physical developer. The selective intensification of Alzheimer's neurofibrillary changes requires a pre-treatment with lanthanum nitrate (10 mM/liter) and 3% hydrogen peroxide for 1 hr. The diaminobenzidine reaction can be selectively intensified when physical development is preceded by consecutive treatments with copper sulfate (10 mM/liter, pH 5, 10 min) and hydrogen peroxide (3%, pH 7, 10 min). In peroxidase histochemistry, this high-grade intensification may help to increase specificity and reduce the threshold of detectability in tracing neurons with horseradish peroxidase or in immunohistochemistry when the peroxidase-antiperoxidase method is used.
物理显影剂可以增强某些组织化学反应终产物的可见性，例如二氨基联苯胺的氧化聚合反应以及碘化银络离子与阿尔茨海默病神经原纤维变化的选择性结合。不幸的是，这种银染增强作用通常叠加在源自嗜银Ⅲ反应的非特异性染色上，当用物理显影剂处理组织切片时，嗜银Ⅲ反应也会发生。本研究表明，在将组织切片转移到物理显影剂之前，先用某些金属离子和过氧化氢处理，可以抑制嗜银Ⅲ反应。阿尔茨海默病神经原纤维变化的选择性增强需要用硝酸镧（10 mM/升）和3%过氧化氢预处理1小时。当先用硫酸铜（10 mM/升，pH 5，10分钟）和过氧化氢（3%，pH 7，10分钟）连续处理后再进行物理显影时，二氨基联苯胺反应可以被选择性增强。在过氧化物酶组织化学中，这种高级别的增强可能有助于提高特异性，并在使用辣根过氧化物酶追踪神经元或使用过氧化物酶-抗过氧化物酶方法进行免疫组织化学时降低可检测性阈值。
