Lun Z R, Desser S S
Department of Zoology, University of Toronto, Ontario, Canada.
J Parasitol. 1995 Dec;81(6):1018-20.
The chromosomes of 12 species and isolates of anuran trypanosomes were investigated by pulsed-field gradient gel electrophoresis. Twelve to 16 chromosomes ranging from 0.45 to 2.2 megabase pairs were found in each of these trypanosomes. Minichromosomes were not observed in any of the examined isolates. Results indicate that different species of anuran trypanosomes display distinct karyotype patterns, and that isolates from the same region are similar. Our findings also reveal that most chromosome profiles of these trypanosomes are in accordance with isoenzyme and random amplified polymorphic DNA analysis data.
通过脉冲场梯度凝胶电泳对12种无尾目锥虫的染色体和分离株进行了研究。在这些锥虫的每一种中都发现了12至16条染色体，大小从0.45到2.2兆碱基对不等。在所检查的任何分离株中均未观察到微型染色体。结果表明，不同种类的无尾目锥虫显示出不同的核型模式，并且来自同一区域的分离株相似。我们的研究结果还表明，这些锥虫的大多数染色体图谱与同工酶和随机扩增多态性DNA分析数据一致。
