Lun Z R, Desser S S
Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong.
Folia Parasitol (Praha). 1996;43(4):241-8.
The patterns of random amplified fragments and molecular karyotypes of 12 isolates of anuran trypanosomes continuously cultured in vitro were compared by random amplified polymorphic DNA (RAPD) analysis and pulsed field gradient gel electrophoresis (PFGE). The time interval between preparation of two series of samples was one year. Changes were not observed in the number and size of sharp, amplified fragments of DNA samples from both series examined with the ten primers used. Likewise, changes in the molecular karyotypes were not detected between the two samples of these isolates. These results suggest that the molecular karyotype and the RAPD patterns of the anuran trypanosomes remain stable after being cultured continuously in vitro for one year.
通过随机扩增多态性DNA（RAPD）分析和脉冲场梯度凝胶电泳（PFGE），比较了在体外连续培养的12株无尾目锥虫分离株的随机扩增片段模式和分子核型。两个系列样品制备的时间间隔为一年。用所使用的10种引物检测的两个系列DNA样品的清晰扩增片段的数量和大小均未观察到变化。同样，在这些分离株的两个样品之间未检测到分子核型的变化。这些结果表明，无尾目锥虫在体外连续培养一年后，其分子核型和RAPD模式保持稳定。
