Bristol Dental School, University of Bristol, Lower Maudlin Street, Bristol, BS1 2LY, UK.
Centre for Cell Engineering, Institute of Molecular Cell and Systems Biology, University of Glasgow, Joseph Black Building, University Avenue, Glasgow, G12 8QQ, UK.
J Mater Sci Mater Med. 2021 Nov 20;32(12):139. doi: 10.1007/s10856-021-06616-5.
Dental implants are an increasingly popular way to replace missing teeth. Whilst implant survival rates are high, a small number fail soon after placement, with various factors, including bacterial contamination, capable of disrupting osseointegration. This work describes the development of chlorhexidine-hexametaphosphate coatings for titanium that hydrolyse to release the antiseptic agent chlorhexidine. The aim was to develop a coating for titanium that released sufficient chlorhexidine to prevent biofilm formation, whilst simultaneously maintaining cytocompatibility with cells involved in osseointegration. The coatings were characterised with respect to physical properties, after which antibiofilm efficacy was investigated using a multispecies biofilm model, and cytocompatibility determined using human mesenchymal stem cells. The coatings exhibited similar physicochemical properties to some implant surfaces in clinical use, and significantly reduced formation of multispecies biofilm biomass up to 72 h. One coating had superior cytocompatibility, with mesenchymal stem cells able to perform normal functions and commence osteoblastic differentiation, although at a slower rate than those grown on uncoated titanium. With further refinement, these coatings may have application in the prevention of bacterial contamination of dental implants at the time of surgery. This could aid a reduction in rates of early implant failure.
种植牙是一种越来越受欢迎的修复缺失牙齿的方法。尽管种植体的存活率很高，但仍有一小部分在植入后不久就失效了，包括细菌污染在内的各种因素都可能破坏骨整合。本研究描述了用于钛的氯己定-六偏磷酸盐水解涂层的开发，该涂层可释放出防腐剂氯己定。目的是开发一种能够释放足够的氯己定以防止生物膜形成的钛涂层，同时保持与骨整合相关细胞的细胞相容性。对涂层的物理性能进行了表征，然后使用多物种生物膜模型研究了其抗生物膜功效，并使用人骨髓间充质干细胞测定了其细胞相容性。该涂层的理化性能与一些临床应用中的种植体表面相似，并且能够在长达 72 小时的时间内显著减少多物种生物膜生物量的形成。一种涂层具有更好的细胞相容性，骨髓间充质干细胞能够正常发挥功能并开始成骨细胞分化，尽管其速度比在未涂层钛上生长的细胞慢。经过进一步改进，这些涂层可能有助于在手术时防止种植牙的细菌污染。这可能有助于降低早期种植体失败的发生率。
