Parada M A, Puig de Parada M, Hernandez L, Hoebel B G
Department of Physiology, Los Andes University School of Medicine, Venezuela.
J Neurosci Methods. 1995 Aug;60(1-2):133-9. doi: 10.1016/0165-0270(95)00004-e.
A low-torque, bubble-free and multiple-channel fluid swivel of easy construction was recently described. This paper describes the design, construction and testing of 3 electrical channels added to the original fluid swivel. The new channels were tested monitoring intrahypothalamic temperature (T(hy)) by means of a copper-constantan thermocouple in freely moving rats, before and after a single intraperitoneal (i.p.) amphetamine injection (3 mg/kg). This test showed an increase in T(hy) after the injection and the maintenance of the electrical continuity along the whole testing period, even when the animals were hyperactive. With this improvement the original swivel was transformed in a more versatile device for experiments requiring fluid handlings and electrophysiological manipulations. Electrical stimulation as in kindling or brain self-stimulation, and electrophysiological recordings as in electroencephalography, electromiography, electrocardiography, in vivo voltammetry and even neuronal unit recording, are just examples of the electrophysiological methods that can be combined with drug self-administration and microdialysis using the present device.
最近描述了一种结构简单的低扭矩、无气泡且多通道的流体旋转接头。本文描述了在原始流体旋转接头上增加3个电通道的设计、构造和测试。在自由活动的大鼠单次腹腔注射（i.p.）苯丙胺（3 mg/kg）之前和之后，通过铜-康铜热电偶监测下丘脑内温度（T(hy)）对新通道进行了测试。该测试表明注射后T(hy)升高，并且即使动物处于多动状态，在整个测试期间电连续性得以维持。通过这一改进，原始旋转接头转变为一种更通用的装置，适用于需要流体处理和电生理操作的实验。诸如点燃或脑自我刺激中的电刺激，以及脑电图、肌电图、心电图、体内伏安法甚至神经元单位记录中的电生理记录，只是可以与使用本装置的药物自我给药和微透析相结合的电生理方法的示例。
