López-Román A, Armengol J A
Departamento de Ciencias Morfológicas, Facultad de Medicina, Universidad de Sevilla, Spain.
J Neurosci Methods. 1995 Aug;60(1-2):39-42. doi: 10.1016/0165-0270(94)00217-5.
Acridine Orange is commonly used as a fluorescent counterstain in fluorescent tract tracing techniques. Here we describe a method in which the substitution of the standard washing solutions (i.e., 0.9% saline) for a diluted solution of Acridine Orange (0.001%) during the perfusion of the animal before fixation provides a fluorescent counterstaining compatible with Fast Blue fluorescent retrograde labeling. In contrast to other fluorescent counterstaining methods, this procedure minimizes the diminution in the fluorescence of the tracer during the handling of sections.
吖啶橙通常用作荧光束追踪技术中的荧光复染剂。在此，我们描述了一种方法，即在固定动物之前进行灌注时，用稀释的吖啶橙溶液（0.001%）替代标准洗涤液（即0.9%生理盐水），可提供与固蓝荧光逆行标记兼容的荧光复染。与其他荧光复染方法相比，该程序可最大程度减少切片处理过程中示踪剂荧光的减弱。
