Smithwick R W, Bigbie M R, Ferguson R B, Karlix M A, Wallis C K
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1995 Oct;33(10):2763-4. doi: 10.1128/jcm.33.10.2763-2764.1995.
A new fluorescence acid-fast staining method with acridine orange as the specific stain is presented. Only two reagents are required: the acridine orange-specific stain and a destaining-counterstaining reagent. Compared with auramine fluorescence acid-fast staining, there was less nonspecific staining of non-acid-fast debris which fluoresced a pale green contrasting color to provide a background in which to search for the red-to-orange fluorescing acid-fast bacilli. The results of the study indicate that the acridine orange method is superior to the auramine method in detecting acid-fast bacilli in specimen smears.
本文介绍了一种以吖啶橙作为特异性染色剂的新型荧光抗酸染色方法。该方法仅需两种试剂：吖啶橙特异性染色剂和脱色复染试剂。与金胺荧光抗酸染色相比，非抗酸碎片的非特异性染色较少，这些碎片发出淡绿色的对比色荧光，形成一个背景，以便在其中寻找发出红橙色荧光的抗酸杆菌。研究结果表明，在检测标本涂片的抗酸杆菌方面，吖啶橙法优于金胺法。
