Kinchington P R, Turse S E
Department of Ophthalmology, University of Pittsburgh, PA 15213, USA.
Neurology. 1995 Dec;45(12 Suppl 8):S13-4. doi: 10.1212/wnl.45.12_suppl_8.s13.
A live attenuated varicella vaccine, derived from a Japanese isolate, is currently being widely used to modulate disease caused by varicella-zoster virus. Differentiation of the vaccine from wild-type isolates has been and will continue to be critical in the assessment of the vaccine in the United States. This has largely relied upon identification of characteristic DNA polymorphisms in the vaccine strain in the United States. This has largely relied upon identification of characteristic DNA polymorphisms in the vaccine strain. In this report, we describe the identification of a new sequence polymorphism, located in the N-terminal coding sequence of open reading frame (ORF) 10. This variation results in the synthesis of an ORF 10 protein that is differentially recognized by antibodies to an ORF 10 synthetic peptide. The variation appears to be completely restricted to Japanese strains, including that used for the live attenuated varicella vaccine. As such, this polymorphism and the antibodies that differentially recognize it could prove highly useful in the assessment of the Japanese vaccine in the United States.
一种源自日本分离株的减毒活水痘疫苗目前正被广泛用于调节水痘-带状疱疹病毒引起的疾病。在美国，区分疫苗株与野生型分离株在疫苗评估中一直并将继续至关重要。这在很大程度上依赖于在美国鉴定疫苗株中特征性的DNA多态性。在本报告中，我们描述了一个位于开放阅读框（ORF）10的N端编码序列中的新序列多态性的鉴定。这种变异导致合成一种ORF 10蛋白，该蛋白被针对ORF 10合成肽的抗体有差异地识别。这种变异似乎完全局限于日本毒株，包括用于减毒活水痘疫苗的毒株。因此，这种多态性以及能有差异地识别它的抗体在美国对日本疫苗的评估中可能会被证明非常有用。
