Welter J F, Eckert R L
Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4970, USA.
Oncogene. 1995 Dec 21;11(12):2681-7.
Activator protein 1 (AP1) family proteins have been implicated in the regulation of genes expressed in the epidermis. However, no comprehensive analysis of the expression patterns of the known AP1 family proteins in the human epidermis or any other terminally differentiating tissue has been performed. In the present study we describe the localization of c-fos, fosB, Fra-1, Fra-2, c-jun, junB and junD in the normal human epidermis. Each is expressed in specific epidermal layers. c-fos is localized in the nuclei of the upper spinous and granular layer cells. FosB is present in the nuclei in all layers. Fra-1 is absent from the basal layer, but is present in all other layers. Fra-2 is detected in all layers, but staining intensity is increased in the upper spinous layer. c-jun staining is limited to the granular layer, while junB and junD are present in all layers. The differentiation-dependent pattern of expression of the AP1 family members suggest an important role for these proteins in specifying the temporal and spatial pattern of gene expression during keratinocyte differentiation.
活化蛋白1（AP1）家族蛋白与表皮中表达的基因调控有关。然而，尚未对已知的AP1家族蛋白在人表皮或任何其他终末分化组织中的表达模式进行全面分析。在本研究中，我们描述了c-fos、fosB、Fra-1、Fra-2、c-jun、junB和junD在正常人类表皮中的定位。它们各自在特定的表皮层中表达。c-fos定位于棘层上部和颗粒层细胞的细胞核中。FosB存在于所有层的细胞核中。Fra-1在基底层中不存在，但在所有其他层中存在。Fra-2在所有层中均有检测到，但在上部棘层中染色强度增加。c-jun染色仅限于颗粒层，而junB和junD存在于所有层中。AP1家族成员的分化依赖性表达模式表明这些蛋白在确定角质形成细胞分化过程中基因表达的时空模式方面具有重要作用。
