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原索动物性腺中促黑素样肽的免疫细胞化学定位及生化特性

Immunocytochemical localization and biochemical characterization of melanotropin-like peptides in the gonads of a protochordate.

作者信息

Pestarino M, Facchinetti F

机构信息

Institute of Comparative Anatomy, University of Genova, Italy.

出版信息

Peptides. 1995;16(7):1269-72. doi: 10.1016/0196-9781(95)02016-p.

DOI:10.1016/0196-9781(95)02016-p
PMID:8545249
Abstract

The compound gonads of the protochordate ascidian Styela plicata were investigated by immunocytochemistry, HPLC, and radioimmunoassay to verify the presence of melanotropin-like peptides, alpha-MSH-like immunoreactivity is localized in the follicular cells and in the perinuclear cytoplasm of different types of ovaric follicles, as well as in the spermatogonia and spermatocytes of testicular lobules. The ascidian immunoreactive peptides occurring in the gonads consist of alpha-MSH and ACTH(1-13)-NH2 and their amounts are higher in summer than in winter.

摘要

通过免疫细胞化学、高效液相色谱法和放射免疫分析法对原索动物皱瘤海鞘的复合性腺进行了研究,以验证是否存在促黑素样肽。α-促黑素样免疫反应性定位于不同类型卵巢滤泡的滤泡细胞和核周细胞质中,以及睾丸小叶的精原细胞和精母细胞中。性腺中出现的海鞘免疫反应性肽由α-促黑素和促肾上腺皮质激素(1-13)-NH2组成,其含量夏季高于冬季。

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