Diallo I S, Bensink J C, Frost A J, Spradbrow P B
Department of Veterinary Pathology, University of Queensland, St. Lucia, Brisbane, Australia.
Vet Microbiol. 1995 Sep;46(1-3):335-42. doi: 10.1016/0378-1135(95)00099-v.
A collection of 45 strains of Pasteurella multocida was assembled. The strains had been isolated from cases of fowl cholera in eastern Australia over 8 years, and included mainly type A strains. All the strains were examined for plasmids and resistance to 10 antimicrobial agents and most of the strains were examined for restriction fragment length polymorphism. Nine strains were assayed for pathogenicity for mice. Twenty strains yielded no plasmid. Seven contained a single plasmid of 1.3 kbp and 18 contained 2 plasmids, of 2.4 and 7.5 kbp. All the strains were resistant to streptomycin, trimethoprim and lincomycin while one strain was resistant to tetracycline. There was no correlation between plasmid content and resistance to antimicrobial agents. Three strains that lacked plasmids were highly virulent for mice, 6 strains containing plasmids were not. Restriction fragment length polymorphism generated by HpaII allowed the 39 strains that were tested to be divided into 10 groups.
收集了45株多杀性巴氏杆菌菌株。这些菌株是在8年多的时间里从澳大利亚东部的禽霍乱病例中分离出来的，主要包括A型菌株。对所有菌株进行了质粒检测以及对10种抗菌剂的耐药性检测，并且对大多数菌株进行了限制性片段长度多态性检测。对9株菌株进行了小鼠致病性测定。20株菌株未检测到质粒。7株含有一个1.3千碱基对的单一质粒，18株含有两个质粒，大小分别为2.4千碱基对和7.5千碱基对。所有菌株对链霉素、甲氧苄啶和林可霉素耐药，而有一株对四环素耐药。质粒含量与对抗菌剂的耐药性之间没有相关性。3株无质粒的菌株对小鼠具有高毒力，6株含质粒的菌株则不然。由HpaII产生的限制性片段长度多态性使接受检测的39株菌株被分为10组。
