Joles J A, Bijleveld C, van Tol A, Geelen M J, Koomans H A
Department of Nephrology and Hypertension, Faculty of Medicine, Utrecht University, The Netherlands.
Atherosclerosis. 1995 Sep;117(1):51-9. doi: 10.1016/0021-9150(95)05557-d.
In mutant analbuminaemic rats (NAR), females demonstrate a more marked hypertriglyceridaemia than males. Ovariectomy decreases triglyceride levels in female NAR. We measured triglyceride secretion rates in vivo as well as the activity of acetyl CoA carboxylase (ACC) and fatty acid synthase (FAS) in hepatic cytosol obtained from female control Sprague-Dawley (SD) rats and NAR with or without ovariectomy. NAR were severely hyperlipidaemic, and triglyceride, cholesterol, apolipoprotein A-I and plasma protein concentrations levels were decreased (all P < 0.01) by ovariectomy. Only triglyceride levels were decreased by ovariectomy in the SD rats (P < 0.05). Oestradiol treatment in ovariectomized NAR restored plasma protein and triglyceride concentrations to levels similar to those observed in intact female NAR and caused a marked increase in plasma cholesterol. Ovariectomy in NAR reduced lipoprotein triglycerides and cholesterol in VLDL, IDL and LDL1, but had little effect on the triglyceride-cholesterol ratio of these particles. Both ACC and FAS activities were markedly increased in NAR vs. SD rats (P < 0.01). This increase was partially corrected by ovariectomy. There was no significant effect of ovariectomy on ACC or FAS activity in the SD rats. Triglyceride secretion rates were significantly increased in NAR vs. SD rats (135 +/- 10 vs. 103 +/- 12 nmol/min per 100 g body weight; P < 0.05). Ovariectomy markedly decreased triglyceride secretion rate in NAR to 69 +/- 6 (P < 0.01), but not in SD rats (92 +/- 8 nmol/min per 100 g body weight, NS). Oestradiol treatment in ovariectomized SD rats restored triglyceride levels but had no significant effect on triglyceride secretion rate (106 +/- 23 nmol/min per 100 g).(ABSTRACT TRUNCATED AT 250 WORDS)
在突变性无白蛋白血症大鼠（NAR）中，雌性大鼠的高甘油三酯血症比雄性更为明显。卵巢切除术可降低雌性NAR的甘油三酯水平。我们测量了体内甘油三酯分泌率，以及从雌性对照Sprague-Dawley（SD）大鼠和有或无卵巢切除术的NAR获得的肝细胞溶质中乙酰辅酶A羧化酶（ACC）和脂肪酸合酶（FAS）的活性。NAR严重高脂血症，卵巢切除术可降低甘油三酯、胆固醇、载脂蛋白A-I和血浆蛋白浓度水平（所有P<0.01）。卵巢切除术仅降低了SD大鼠的甘油三酯水平（P<0.05）。去卵巢NAR接受雌二醇治疗后，血浆蛋白和甘油三酯浓度恢复到与完整雌性NAR相似的水平，并导致血浆胆固醇显著升高。NAR的卵巢切除术降低了VLDL、IDL和LDL1中的脂蛋白甘油三酯和胆固醇，但对这些颗粒的甘油三酯-胆固醇比值影响不大。与SD大鼠相比，NAR中ACC和FAS活性均显著增加（P<0.01）。卵巢切除术部分纠正了这种增加。卵巢切除术对SD大鼠的ACC或FAS活性无显著影响。与SD大鼠相比，NAR的甘油三酯分泌率显著增加（135±10对103±12 nmol/min每100 g体重；P<0.05）。卵巢切除术使NAR的甘油三酯分泌率显著降低至69±6（P<0.01），但对SD大鼠无影响（92±8 nmol/min每100 g体重，无显著性差异）。去卵巢SD大鼠接受雌二醇治疗后甘油三酯水平恢复，但对甘油三酯分泌率无显著影响（106±23 nmol/min每100 g）。（摘要截短至250字）
