Tikoo S K, Campos M, Popowych Y I, van Drunen Littel-van den Hurk S, Babiuk L A
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Viral Immunol. 1995;8(1):19-25. doi: 10.1089/vim.1995.8.19.
The lymphocyte proliferative response to BHV-1 in immune cattle was compared to recombinant wild-type gD and truncated gD produced from recombinant vaccinia viruses. The response exhibited by recombinant proteins was comparable to the response induced by BHV-1 suggesting that gD is the major target structure for stimulation of bovine lymphocytes. Analysis of the proliferative response using vaccinia virus vectors expressing various modified forms of gD identified a region between residues 165 and 216 recognized by T-lymphocytes of immune cattle. Further analysis by overlapping peptides in this region localized the T cell epitope to residues 161-172. Antibody-blocking studies demonstrated that lymphocytes responding to this epitope are CD4+. In addition, lymphocytes stimulated with gD or peptide 77 (residues 161-172) also produced IFN-gamma and IL-2.
将免疫牛对牛疱疹病毒1型（BHV-1）的淋巴细胞增殖反应与重组野生型gD和重组痘苗病毒产生的截短型gD进行了比较。重组蛋白表现出的反应与BHV-1诱导的反应相当，这表明gD是刺激牛淋巴细胞的主要靶结构。使用表达各种修饰形式gD的痘苗病毒载体对增殖反应进行分析，确定了免疫牛T淋巴细胞识别的165至216位残基之间的区域。通过该区域的重叠肽进一步分析将T细胞表位定位到161-172位残基。抗体阻断研究表明，对该表位作出反应的淋巴细胞是CD4+。此外，用gD或肽77（161-172位残基）刺激的淋巴细胞也产生γ干扰素和白细胞介素-2。
