Briz M, Cabrera R, Sanjuán I, Forés R, Díez J L, Herrero M, Regidor C, Algora M, Fernández M N
Department of Haematology, Hospital Puerta de Hierro, Universidad Autónoma de Madrid, Spain.
Br J Haematol. 1995 Oct;91(2):409-11. doi: 10.1111/j.1365-2141.1995.tb05311.x.
Transfusion-associated graft-versus-host disease (TA-GVHD), has rarely been reported associated with B-chronic lymphocytic leukaemia (B-CLL). We report a patient diagnosed with B-CLL, previously treated with fludarabine, who developed TA-GVHD after being transfused during surgery for splenectomy. Diagnosis was confirmed by polymerase chain reaction (PCR) detection of donor DNA in the patient, by amplification of Y-chromosome sequence and analysis of minisatellite polymorphisms. B-CLL patients treated with fludarabine appear to be at risk for TA-GVHD and should be regarded as candidates for transfusions with irradiated blood products. This case illustrates that PCR is a rapid technique for the early diagnosis of TA-GVHD.
输血相关移植物抗宿主病（TA-GVHD）很少被报道与B细胞慢性淋巴细胞白血病（B-CLL）相关。我们报告了一名被诊断为B-CLL的患者，此前接受过氟达拉滨治疗，该患者在脾切除手术输血后发生了TA-GVHD。通过聚合酶链反应（PCR）检测患者体内的供体DNA、扩增Y染色体序列以及分析微卫星多态性，确诊了该病。接受氟达拉滨治疗的B-CLL患者似乎有发生TA-GVHD的风险，应被视为输注辐照血液制品的候选对象。本病例表明，PCR是早期诊断TA-GVHD的快速技术。
