Delon J, Legendre P
INSERM U261, Département des Biotechnologies, Institut Pasteur, Paris, France.
Neuroreport. 1995 Oct 2;6(14):1932-6. doi: 10.1097/00001756-199510020-00026.
We examined the effect of altering the cytoskeleton polymerization state by treatment with nocodazole and taxol on glycine-evoked currents in patch-clamp recordings from cultured spinal cord neurones. Adding ATP and GTP to the pipette solution did not prevent the rundown of the peak current. In the absence or in the presence of ATP, the proportion of the non-desensitizing part of the glycine evoked-current declined with time. Adding intracellular GTP and ATP stabilized glycine-evoked responses although the proportion of non-inactivating current was reduced. Nocodazole reduced by itself the proportion of the non-inactivating current whereas taxol (with ATP and GTP) had an opposite effect. These results suggest that the polymerization state of microtubules has functional consequences on glycine receptors.
我们通过用诺考达唑和紫杉醇处理来改变细胞骨架聚合状态，研究其对培养的脊髓神经元膜片钳记录中甘氨酸诱发电流的影响。向吸管溶液中添加ATP和GTP并不能阻止峰值电流的衰减。在不存在或存在ATP的情况下，甘氨酸诱发电流的非脱敏部分比例随时间下降。添加细胞内GTP和ATP可稳定甘氨酸诱发的反应，尽管非失活电流的比例降低。诺考达唑自身会降低非失活电流的比例，而紫杉醇（与ATP和GTP一起）则有相反的作用。这些结果表明微管的聚合状态对甘氨酸受体有功能影响。
