Peraçoli J C, Fortes M R, Rudge M V, Rezkallah-Iwasso M T, Peraçoli M T
Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, Brasil.
Braz J Med Biol Res. 1995 Jun;28(6):655-61.
The number and activity of natural killer (NK) cells were studied in 20 patients with pregnancy-induced hypertension (PIH), 15 uncomplicated pregnant women and 16 healthy non-pregnant women. All the pregnant women were primigravidae and were evaluated during the third trimester of gestation. Peripheral blood NK cells were detected with monoclonal antibodies by indirect immunofluorescence and cytotoxic activity was measured using a single-cell assay against K562 target cells. Hypertensive pregnant women had an increased number of circulating NK cells associated with a significant decrease of NK activity. The cytotoxic activity was significantly lower in normal pregnant and PIH women when compared with non-pregnant controls. The onset of immature NK cells in peripheral blood and the impairment of their cytotoxic activity in PIH patients may be associated with hormones and immunosuppressive substances produced by tissues occurring at the maternal-fetal interface.
对20例妊娠高血压综合征（PIH）患者、15例无并发症孕妇及16例健康非孕妇女的自然杀伤（NK）细胞数量及活性进行了研究。所有孕妇均为初产妇，并在妊娠晚期进行评估。采用间接免疫荧光法用单克隆抗体检测外周血NK细胞，并用针对K562靶细胞的单细胞试验测定细胞毒性活性。妊娠高血压妇女循环NK细胞数量增加，同时NK活性显著降低。与非孕对照组相比，正常孕妇和PIH妇女的细胞毒性活性显著降低。PIH患者外周血中未成熟NK细胞的出现及其细胞毒性活性的损害可能与母胎界面组织产生的激素和免疫抑制物质有关。
