采用安息香进行柱前荧光衍生化，用自动高效液相色谱法测定人血清中的神经氨酸酶抑制剂（GG167）

Automated high-performance liquid chromatographic method for the determination of a neuraminidase inhibitor (GG167) in human serum by pre-column fluorescence derivatisation using benzoin.

作者信息

Stubbs R J, Harker A J

机构信息

Glaxo Research and Development Ltd, Greenford, Middlesex, UK.

出版信息

J Chromatogr B Biomed Appl. 1995 Aug 18;670(2):279-85. doi: 10.1016/0378-4347(95)00165-4.

DOI:10.1016/0378-4347(95)00165-4

Abstract

A pre-column fluorescence derivatisation high-performance liquid chromatographic method for the analysis of a neuraminidase inhibitor, GG167, in human serum is described. GG167 was extracted from serum samples using Bond Elut SCX solid-phase extraction cartridges, followed by derivatisation with benzoin prior to reversed-phase chromatography with fluorescence detection. This method has been automated using a Zymark robot and used in the analysis of human serum samples from clinical studies. The method has been shown to be valid over a concentration range of 10-800 ng/ml using a 1-ml sample volume.

摘要

本文描述了一种用于分析人血清中神经氨酸酶抑制剂GG167的柱前荧光衍生化高效液相色谱法。使用Bond Elut SCX固相萃取柱从血清样品中提取GG167，然后在进行反相色谱荧光检测之前用苯偶姻进行衍生化。该方法已使用Zymark机器人实现自动化，并用于临床研究中人类血清样品的分析。使用1 ml样品体积时，该方法在10 - 800 ng/ml的浓度范围内已被证明是有效的。

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