Weir J P, Bennett M, Allen E M, Elkins K L, Martin S, Rouse B T
Department of Microbiology, University of Tennessee, Knoxville 37996.
J Gen Virol. 1989 Oct;70 ( Pt 10):2587-94. doi: 10.1099/0022-1317-70-10-2587.
The gene encoding the herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) was isolated and cloned into a vaccinia virus insertion vector, and the resulting vaccinia-gC vector was used to construct a recombinant vaccinia virus that expressed gC (VVgC5). Infection of cells with VVgC5 resulted in cell surface expression of authentic HSV-1 gC. HSV-1 gC-specific neutralizing antibodies were produced in VVgC5-immunized mice, and lymphocytes exhibited an HSV-1-specific proliferation response in vitro following infection. More importantly, VVgC5-immunized mice were resistant to subsequent lethal HSV-1 challenge.
编码单纯疱疹病毒1型（HSV-1）糖蛋白C（gC）的基因被分离并克隆到痘苗病毒插入载体中，所得的痘苗-gC载体用于构建表达gC的重组痘苗病毒（VVgC5）。用VVgC5感染细胞导致细胞表面表达真实的HSV-1 gC。在经VVgC5免疫的小鼠中产生了HSV-1 gC特异性中和抗体，并且淋巴细胞在感染后体外表现出HSV-1特异性增殖反应。更重要的是，经VVgC5免疫的小鼠对随后的致死性HSV-1攻击具有抗性。
