Rivest R, Falardeau P, Di Paolo T
School of Pharmacy, Laval University, Quebec, Canada.
Brain Res. 1995 Sep 18;692(1-2):269-72. doi: 10.1016/0006-8993(95)00611-s.
Gender differences and the effect of chronic haloperidol on the rat brain dopamine transporter is reported. The density of striatal dopamine transporter sites labelled with [3H]GBR 12935, and of substantia nigra dopamine transporter mRNA measured by in situ hybridization were higher in female compared to male rats whereas striatal D2 specific binding labelled with [3H]spiperone was not significantly higher. Daily haloperidol treatment (1 mg/kg, i.p.) for 21 days increased striatal [3H]spiperone specific binding but left unchanged striatal [3H]GBR 12935 binding density and affinity as well as substantia nigra dopamine transporter mRNA levels. A reduce clearance rate of dopamine in the striatum after acute and chronic haloperidol was previously reported; the present results indicate that this may occur without changes in the sites of dopamine transport or in gene expression of this transporter.
报告了性别差异以及慢性给予氟哌啶醇对大鼠脑多巴胺转运体的影响。与雄性大鼠相比，用[3H]GBR 12935标记的纹状体多巴胺转运体位点密度以及通过原位杂交测量的黑质多巴胺转运体mRNA在雌性大鼠中更高，而用[3H]螺哌隆标记的纹状体D2特异性结合则没有显著更高。每天腹腔注射氟哌啶醇（1 mg/kg），持续21天，增加了纹状体[3H]螺哌隆特异性结合，但纹状体[3H]GBR 12935结合密度和亲和力以及黑质多巴胺转运体mRNA水平未发生变化。先前报道急性和慢性给予氟哌啶醇后纹状体中多巴胺清除率降低；目前的结果表明，这可能在多巴胺转运位点或该转运体基因表达无变化的情况下发生。
