Guzikevich-Guerstein G, Shakked Z
Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
Nat Struct Biol. 1996 Jan;3(1):32-7. doi: 10.1038/nsb0196-32.
The structure of the TATA-box bound to the TATA-binding protein revealed a new and unexpected deformation of the double helix leading to a sharp change in the DNA trajectory. Here we show that the deformation imposed upon the TATA-box represents a novel form of the double helix--named TA-DNA--which differs from A-DNA by a single conformational parameter, namely the rotation around the glycosidic bond. This rotation causes a 50 degrees inclination of the base pairs in the TATA-box which in turn results in abrupt change in the trajectory of the flanking B-DNA segments. The observation that the TATA sequence can assume an A-DNA conformation coupled to the simplicity of the transition from A-DNA to TA-DNA may be the reason for the presence of the TATA sequence in a wide range of promoters.
与TATA结合蛋白结合的TATA框结构揭示了双螺旋结构一种新的意外变形，导致DNA轨迹发生急剧变化。我们在此表明，施加于TATA框的变形代表了双螺旋的一种新形式——命名为TA-DNA——它与A-DNA仅在一个构象参数上不同，即围绕糖苷键的旋转。这种旋转导致TATA框中碱基对倾斜50度，进而导致侧翼B-DNA片段的轨迹突然改变。TATA序列可呈现A-DNA构象以及从A-DNA到TA-DNA转变的简单性这一观察结果，可能是TATA序列存在于广泛启动子中的原因。
