Zeghal N, Redjem M, Gondran F, Vigouroux E
Laboratoire de Physiologie Animale, Faculté des Sciences de Sfax, Tunisie, France.
Arch Physiol Biochem. 1995 Aug;103(4):502-11. doi: 10.3109/13813459509047146.
In the suckling and adult rats equilibrated or no by 125I, cutaneous iodine analysed by dialysis and chromatography techniques (Dowex and Sephadex) was the purpose of this study. Dialysis studies had shown that most of steady or labelled skin iodine had an iodide form (90 to 95% of the total iodine). There were at least two intracellular iodide pools: the first one was quickly dialysable, in fact about 60 percent of initial radioactivity represented the intracellular iodide equilibrated with extracellular fluid. The other one wasn't or was little dialysable representing probably the cutaneous iodide storage compartment. Chromatography studies (Dowex or Sephadex) demonstrated that the skin of the young and adult rat contained T4 and T3 hormones in a small percentage. The iodide represented a value more than 90% of initial total radioactivity. Results concerning kinetic skin iodine, eight and twenty four hours after LT4(125)I injected to adults and to control and iodine deficient ten day old rats, confirmed those obtained here by Dowex chromatography and previous ones. Triiodothyronine (T3) might have an origin either in thyroid synthesis or in deiodination of T4. Consequently we may say that the skin of the 10 day and 14 day old rats presented a great accumulation of iodide. The perchlorate inhibited this storage. Indeed, in young deficient iodine rats at birth, cutaneous iodide concentrations were reduced whereas those of T4 and T3 as well as the ratio T3/T4 haven't been modified. Therefore the iodine deficiency seemed to have a few effects on the skin deiodinating activity. Indeed the skin of iodine deficient immature rat became unable to accumulate iodide. The main effect of the skin iodine deficiency was the inhibition of iodide transport from the extracellular fluid to the intracellular one, but not inside cellular structures.
本研究旨在通过透析和色谱技术（Dowex和Sephadex）分析经125I平衡或未平衡的哺乳和成年大鼠皮肤中的碘。透析研究表明，大部分稳定或标记的皮肤碘呈碘化物形式（占总碘的90%至95%）。至少有两个细胞内碘池：第一个可快速透析，实际上约60%的初始放射性代表与细胞外液平衡的细胞内碘化物。另一个不可透析或几乎不可透析，可能代表皮肤碘储存区室。色谱研究（Dowex或Sephadex）表明，幼年和成年大鼠的皮肤中含有少量的T4和T3激素。碘化物占初始总放射性的比例超过90%。给成年大鼠、对照大鼠和缺碘10日龄大鼠注射LT4(125)I后8小时和24小时，有关皮肤碘动力学的结果证实了通过Dowex色谱法在此获得的结果以及之前的结果。三碘甲状腺原氨酸（T3）可能来源于甲状腺合成或T4的脱碘。因此，我们可以说10日龄和14日龄大鼠的皮肤呈现出大量碘化物的积累。高氯酸盐抑制了这种储存。事实上，在出生时缺碘的幼年大鼠中，皮肤碘化物浓度降低，而T4和T3的浓度以及T3/T4比值未发生改变。因此，碘缺乏似乎对皮肤脱碘活性影响不大。事实上，缺碘未成熟大鼠的皮肤无法积累碘化物。皮肤碘缺乏的主要影响是抑制碘化物从细胞外液向细胞内的转运，但不影响细胞结构内部。
