Hay B A, Wassarman D A, Rubin G M
Howard Hughes Medical Institute, University of California, Berkeley 94720-3200, USA.
Cell. 1995 Dec 29;83(7):1253-62. doi: 10.1016/0092-8674(95)90150-7.
Apoptotic cell death is a mechanism by which organisms eliminate superfluous or harmful cells. Expression of the cell death regulatory protein REAPER (RPR) in the developing Drosophila eye results in a small eye owing to excess cell death. We show that mutations in thread (th) are dominant enhancers of RPR-induced cell death and that th encodes a protein homologous to baculovirus inhibitors of apoptosis (IAPs), which we call Drosophila IAP1 (DIAP1). Overexpression of DIAP1 or a related protein, DIAP2, in the eye suppresses normally occurring cell death as well as death due to overexpression of rpr or head involution defective. IAP death-preventing activity localizes to the N-terminal baculovirus IAP repeats, a motif found in both viral and cellular proteins associated with death prevention.
凋亡性细胞死亡是生物体清除多余或有害细胞的一种机制。在发育中的果蝇眼睛中，细胞死亡调节蛋白收割者（REAPER，RPR）的表达会因细胞过度死亡而导致眼睛变小。我们发现，“线索”（thread，th）基因的突变是RPR诱导的细胞死亡的显性增强子，并且th编码一种与杆状病毒凋亡抑制因子（IAPs）同源的蛋白质，我们将其称为果蝇IAP1（DIAP1）。在眼睛中过表达DIAP1或一种相关蛋白DIAP2，可抑制正常发生的细胞死亡以及因rpr过表达或头部内卷缺陷导致的细胞死亡。IAP的死亡预防活性定位于N端杆状病毒IAP重复序列，这是一种在与死亡预防相关的病毒和细胞蛋白中都存在的基序。
