Lukowitz W, Mayer U, Jürgens G
Institut für Genetik und Mikrobiologie, Lehrstuhl für Genetik, Universität München, Federal Republic of Germany.
Cell. 1996 Jan 12;84(1):61-71. doi: 10.1016/s0092-8674(00)80993-9.
The embryo of the flowering plant Arabidopsis develops by a regular pattern of cell divisions and cell shape changes. Mutations in the KNOLLE (KN) gene affect the rate and plane of cell divisions as well as cell morphology, resulting in mutant seedlings with a disturbed radial organization of tissue layers. At the cellular level, mutant embryos are characterized by incomplete cross walls and enlarged cells with polyploid nuclei. The KN gene was isolated by positional cloning. The predicted KN protein has similarity to syntaxins, a protein family involved in vesicular trafficking. During embryogenesis, KN transcripts are detected in patches of single cells or small cell groups. Our results suggest a function for KN in cytokinesis.
开花植物拟南芥的胚胎通过细胞分裂和细胞形状变化的规则模式发育。KNOLLE（KN）基因突变会影响细胞分裂的速率和平面以及细胞形态，导致突变幼苗的组织层径向组织紊乱。在细胞水平上，突变胚胎的特征是横壁不完整，细胞增大且具有多倍体细胞核。通过定位克隆分离出了KN基因。预测的KN蛋白与 syntaxins 相似，syntaxins 是一个参与囊泡运输的蛋白质家族。在胚胎发生过程中，在单个细胞或小细胞群中检测到KN转录本。我们的结果表明KN在胞质分裂中具有功能。
