Lee S F, Liang Y C, Lin J K
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
Chem Biol Interact. 1995 Dec 22;98(3):283-301. doi: 10.1016/0009-2797(95)03652-0.
Autooxidation of polyphenolic metabolites of benzene, such as hydroquinone (HQ), catechol (CT), 1,2,4-benzenetriol (BT) and pyrogallol (PG), produced several kinds of active oxygen species (AOS). BT and PG induced DNA breaks in the absence of metal ions, especially when producing AOS such as H2O2, O2-, HO. or 1 delta gO2. HQ and CT did not result in double-strand DNA breaks, except when ferrous ion was added, indicating the participation of the Fenton reaction. Polyphenolic fractions isolated from green tea (GTP) exerted inhibitory effects on the autooxidation of BT and suppressive effects on H2O2 or HO. generated from phenolic metabolites of benzene in the presence of S9 or an in vivo system. Additionally, although the activities of antioxidant and phase II enzymes were elevated by both GTP and phenolic metabolites of benzene, GTP counteracted the lowering GSH caused by phenolic metabolites of benzene in rat liver. The above results suggest that GTP and phenolic metabolites of benzene are antagonistic in their response to AOS, especially hydroxyl radical.
苯的多酚类代谢产物如对苯二酚（HQ）、邻苯二酚（CT）、1,2,4-苯三酚（BT）和连苯三酚（PG）的自氧化产生了几种活性氧物种（AOS）。BT和PG在没有金属离子的情况下会诱导DNA断裂，尤其是在产生诸如H2O2、O2-、HO·或1ΔgO2等AOS时。HQ和CT不会导致双链DNA断裂，除非添加亚铁离子，这表明芬顿反应的参与。从绿茶中分离出的多酚组分（GTP）对BT的自氧化具有抑制作用，并且在存在S9或体内系统的情况下，对苯的酚类代谢产物产生的H2O2或HO·具有抑制作用。此外，尽管GTP和苯的酚类代谢产物均可提高抗氧化酶和II期酶的活性，但GTP可抵消苯的酚类代谢产物导致的大鼠肝脏中谷胱甘肽（GSH）含量的降低。上述结果表明，GTP和苯的酚类代谢产物在对AOS（尤其是羟基自由基）的反应中具有拮抗作用。
