Maier C E, Miller R H
Department of Anatomy, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Dev Neurosci. 1995;17(3):149-59. doi: 10.1159/000111283.
The origin of astrocytes and oligodendrocytes in the frog spinal cord, and the factors that regulate their differentiation are currently unknown. To determine the timing and pattern of glial cell differentiation, spinal cord sections from Xenopus at different developmental stages were labeled with antibodies specific for astrocytes and oligodendrocytes. Initially, radially oriented glial cells are present in all spinal cord quadrants and their processes contain similar levels of GFAP+ intermediate filaments in both gray and white matter. These cells then appear to differentiate directly into regionally specialized radially oriented astrocytes. Oligodendrocytes labeled with the 'Olig' antibody, however, are first detectable in the ventral quadrant. Differentiation subsequently occurs in a ventral-to-dorsal sequence beginning at the interface between the gray and white matte. These data suggest that the origin and regulation of Xenopus astrocyte and oligodendrocyte differentiation are distinct.
青蛙脊髓中星形胶质细胞和少突胶质细胞的起源以及调节它们分化的因素目前尚不清楚。为了确定神经胶质细胞分化的时间和模式，用针对星形胶质细胞和少突胶质细胞的特异性抗体标记不同发育阶段非洲爪蟾的脊髓切片。最初，放射状排列的神经胶质细胞存在于所有脊髓象限中，并且它们的突起在灰质和白质中含有相似水平的GFAP +中间丝。这些细胞随后似乎直接分化为区域特化的放射状星形胶质细胞。然而，用“Olig”抗体标记的少突胶质细胞首先在腹侧象限中被检测到。随后分化以腹侧到背侧的顺序发生，从灰质和白质之间的界面开始。这些数据表明非洲爪蟾星形胶质细胞和少突胶质细胞分化的起源和调节是不同的。
