Fukasawa M, Adachi H, Hirota K, Tsujimoto M, Arai H, Inoue K
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Exp Cell Res. 1996 Jan 10;222(1):246-50. doi: 10.1006/excr.1996.0030.
Scavenger receptors mediate the recognition of a wide range of negatively charged macromolecules such as acetylated low-density lipoproteins (AcLDL). We previously reported the isolation of CHO cell variants expressing a novel type of scavenger receptor, distinct from type I and II macrophage scavenger receptors, that recognize both AcLDL and negatively charged liposomes. In the present study, we found that these isolated CHO cells express class B scavenger receptors (SRB1), which have recently been identified as a new member of the CD36 family of membrane proteins. The isolated CHO cells and CHO transfectants constitutively expressing SRB1 recognized and mediated the endocytosis of cells undergoing apoptosis as well as that of negatively charged liposomes. In contrast, CHO cells constitutively expressing type I or II scavenger receptors were unable to take up these ligands.
清道夫受体介导对多种带负电荷大分子的识别，如乙酰化低密度脂蛋白（AcLDL）。我们之前报道过分离出表达新型清道夫受体的CHO细胞变体，该受体不同于I型和II型巨噬细胞清道夫受体，能识别AcLDL和带负电荷的脂质体。在本研究中，我们发现这些分离出的CHO细胞表达B类清道夫受体（SRB1），其最近被鉴定为膜蛋白CD36家族的新成员。分离出的CHO细胞和组成性表达SRB1的CHO转染细胞识别并介导凋亡细胞以及带负电荷脂质体的内吞作用。相比之下，组成性表达I型或II型清道夫受体的CHO细胞无法摄取这些配体。
