Zacchetti D, Peränen J, Murata M, Fiedler K, Simons K
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
FEBS Lett. 1995 Dec 27;377(3):465-9. doi: 10.1016/0014-5793(95)01396-2.
VIP17 is a proteolipid enriched in the CHAPS-insoluble complexes from MDCK cells, and a candidate component of the molecular machinery responsible for the sorting and targeting of proteins to the apical surface. Cloning and sequencing of the cDNA encoding the protein revealed that it is the canine homolog of the human and rat MAL proteins. Analysis by immunofluorescence microscopy of epitope-tagged VIP17/MAL expressed transiently in BHK cells and stably in MDCK cells revealed a perinuclear, vesicular, and plasmalemmal staining. In MDCK cells the distribution was mainly in vesicular structures in the apical cytoplasm. These and other results suggest that VIP17/MAL is an important component in vesicular trafficking cycling between the Golgi complex and the apical plasma membrane.
VIP17是一种富含于MDCK细胞中CHAPS不溶性复合物的蛋白脂质，是负责蛋白质分选并靶向至顶端表面的分子机制的候选组分。编码该蛋白的cDNA的克隆和测序显示，它是人和大鼠MAL蛋白的犬类同源物。通过免疫荧光显微镜分析在BHK细胞中瞬时表达以及在MDCK细胞中稳定表达的表位标记的VIP17/MAL，显示出核周、囊泡状和质膜染色。在MDCK细胞中，其分布主要在顶端细胞质的囊泡结构中。这些以及其他结果表明，VIP17/MAL是在高尔基体复合物和顶端质膜之间循环的囊泡运输中的重要组分。
