Wilson S H, Bailey A M, Nourse C R, Mattei M G, Byrne J A
Molecular Oncology Laboratory, Oncology Research Unit, The Children's Hospital at Westmead, Locked Bag 4001, Westmead, NSW, 2145, Australia.
Genomics. 2001 Aug;76(1-3):81-8. doi: 10.1006/geno.2001.6610.
The TPD52 (tumor protein D52)-like proteins are small coiled-coil motif-bearing proteins which were first identified though their expression in human breast carcinoma. TPD52-like proteins are known to interact in hetero-and homomeric fashions, but there are no known heterologous binding partners for these proteins. We now report the cloning of a novel member of the MAL proteolipid family, named MAL2, though its interaction with a TPD52L2 bait in a yeast two-hybrid screen. MAL2 is predicted to be 176 residues (19 kDa) with four transmembrane domains and is 35.8% identical to MAL, a proteolipid required in apical vesicle transport. The MAL2 prey bound all TPD52-like baits tested in the yeast two-hybrid system and in vitro translation of MAL2 produced a single 19-kDa (35)S-labeled protein which specifically bound full-length GST-Tpd52 in GST pull-down assays. The gene MAL2, which was localized to human chromosomal band 8q23 and shown to consist of four exons, is predominantly expressed in human kidney, lung, and liver. Our study has therefore identified a novel member of the MAL proteolipid family and potentially implicates TPD52-like proteins in vesicle transport.
TPD52（肿瘤蛋白D52）样蛋白是一类带有小的卷曲螺旋基序的蛋白，最初是通过它们在人乳腺癌中的表达而被鉴定出来的。已知TPD52样蛋白以异源和同源的方式相互作用，但目前还没有这些蛋白已知的异源结合伴侣。我们现在报告通过在酵母双杂交筛选中与TPD52L2诱饵相互作用，克隆了MAL蛋白脂质家族的一个新成员，命名为MAL2。预测MAL2有176个氨基酸残基（19 kDa），含有四个跨膜结构域，与MAL有35.8%的同源性，MAL是顶端囊泡运输所需的一种蛋白脂质。在酵母双杂交系统中，MAL2猎物与所有测试的TPD52样诱饵结合，并且MAL2的体外翻译产生了一种单一的19 kDa（35）S标记蛋白，该蛋白在GST下拉实验中能特异性结合全长GST-Tpd52。基因MAL2定位于人类染色体8q23带，由四个外显子组成，主要在人肾、肺和肝中表达。因此，我们的研究鉴定出了MAL蛋白脂质家族的一个新成员，并可能表明TPD52样蛋白参与囊泡运输。
