枯草芽孢杆菌芽孢形成抑制剂SinR与其拮抗剂SinI复合物的结晶。
Crystallisation of the Bacillus subtilis sporulation inhibitor SinR, complexed with its antagonist, SinI.
作者信息
Lewis R J, Brannigan J A, Smith I, Wilkinson A J
机构信息
Department of Chemistry, University of York, Heslington, UK.
出版信息
FEBS Lett. 1996 Jan 2;378(1):98-100. doi: 10.1016/0014-5793(95)01432-2.
The transcription factor SinR, a pleiotropic regulator of late growth processes in Bacillus subtilis, has been crystallised as a complex with its antagonist SinI, in a form suitable for structural analysis. The SinI:SinR crystals diffract X-rays generated from a rotating copper anode source to 2.3 A spacing and a complete native dataset has been collected to this resolution limit. The space group of the crystals is P3(1)21 (or its enantiomorph P3(2)21) with cell dimensions a = b = 60.76 A, c = 87.79 A. Assuming that there is a single SinI:SinR heterodimer in the asymmetric unit, the crystals have a Vm of 2.53 A3.Da-1.
转录因子SinR是枯草芽孢杆菌后期生长过程的多效性调节因子，它已与拮抗剂SinI形成复合物结晶，其形式适合进行结构分析。SinI:SinR晶体将旋转铜阳极源产生的X射线衍射至2.3 Å间距，并已收集到该分辨率极限下的完整天然数据集。晶体的空间群为P3(1)21（或其对映体P3(2)21），晶胞尺寸a = b = 60.76 Å，c = 87.79 Å。假设不对称单元中有一个SinI:SinR异二聚体，晶体的Vm为2.53 Å3.Da-1。
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