与重复序列DNA复合的微小F质粒复制起始蛋白（RepE54）的结晶及初步X射线衍射研究。
Crystallization and preliminary X-ray diffraction studies of a replication initiator protein (RepE54) of the mini-F plasmid complexed with iteron DNA.
作者信息
Komori H, Sasai N, Matsunaga F, Wada C, Miki K
机构信息
Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
出版信息
J Biochem. 1999 Jan;125(1):24-6. doi: 10.1093/oxfordjournals.jbchem.a022262.
A replication initiator protein (RepE54) complexed with iteron DNA at its binding site was crystallized by the hanging drop vapor diffusion method. The crystals belong to monoclinic space group C2 with unit cell dimensions of a = 108.4 A, b = 81.9 A, c = 73.9 A, and beta = 121.5 degrees, where one molecule of the protein-DNA complex exists per asymmetric unit. They diffract X-rays up to 2.6 A resolution with synchrotron radiation.
一种与迭代子DNA在其结合位点复合的复制起始蛋白（RepE54）通过悬滴气相扩散法结晶。晶体属于单斜晶系空间群C2，晶胞参数为a = 108.4 Å，b = 81.9 Å，c = 73.9 Å，β = 121.5°，每个不对称单元中存在一个蛋白质-DNA复合物分子。它们在同步辐射下可将X射线衍射到2.6 Å的分辨率。
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