Pieretti M, Cavalieri C, Conway P S, Gallion H H, Powell D E, Turker M S
Department of Pathology and Laboratory Medicine, University of Kentucky, Lexington 40536, USA.
Int J Cancer. 1995 Dec 20;64(6):434-40. doi: 10.1002/ijc.2910640614.
Seventy-four sporadic ovarian tumors were studied for loss of heterozygosity (LOH) and microsatellite instability (MI) with 20 polymorphic markers on chromosome 17 and at least I marker on every other chromosome. Additionally, activation of the K-ras oncogene was examined through mutation analysis of codon 12. A majority of the tumors analyzed were low grade and/or of the mucinous histologic type. A negative correlation between LOH on chromosome 17 and K-ras activation was observed, with the former alteration present in the majority of high grade serous and endometrioid tumors and the latter most commonly found in the mucinous and low malignant potential (LMP) tumors. In 60% of cases where LOH on chromosome 17 was present, it was observed at all informative markers, indicating chromosome loss. In these cases, frequent events of LOH were observed on the other chromosomes. When confined events of LOH were observed on chromosome 17, fewer events of LOH were observed on the other chromosomes. In the absence of LOH on chromosome 17, LOH on other chromosomes was rare. K-ras activation was most commonly observed in tumors with no LOH events. Two endometrioid tumors and 2 mucinous tumors demonstrated MI. Our data support the involvement of different molecular pathways in the development of different types of ovarian tumors.
对74例散发性卵巢肿瘤进行了杂合性缺失（LOH）和微卫星不稳定性（MI）研究，使用了17号染色体上的20个多态性标记以及其他每条染色体上至少1个标记。此外，通过对密码子12的突变分析检测了K-ras癌基因的激活情况。分析的大多数肿瘤为低级别和/或黏液性组织学类型。观察到17号染色体上的LOH与K-ras激活之间呈负相关，前一种改变存在于大多数高级别浆液性和子宫内膜样肿瘤中，而后一种最常见于黏液性和低恶性潜能（LMP）肿瘤中。在17号染色体存在LOH的60%的病例中，在所有信息性标记处均观察到LOH，表明存在染色体丢失。在这些病例中，在其他染色体上观察到频繁的LOH事件。当在17号染色体上观察到局限性的LOH事件时，在其他染色体上观察到的LOH事件较少。在17号染色体不存在LOH的情况下，其他染色体上的LOH很少见。K-ras激活最常见于无LOH事件的肿瘤中。2例子宫内膜样肿瘤和2例黏液性肿瘤表现出MI。我们的数据支持不同分子途径参与不同类型卵巢肿瘤的发生发展。
