瑞士乳杆菌CNRZ32中二肽酶A基因（pepDA）的测序、分布及失活
Sequencing, distribution, and inactivation of the dipeptidase A gene (pepDA) from Lactobacillus helveticus CNRZ32.
作者信息
Dudley E G, Husgen A C, He W, Steele J L
机构信息
Department of Bacteriology, University of Wisconsin-Madison 53706, USA.
出版信息
J Bacteriol. 1996 Feb;178(3):701-4. doi: 10.1128/jb.178.3.701-704.1996.
Abstract
Previously, the gene for a general dipeptidase (pepDA) was isolated from a gene bank of Lactobacillus helveticus CNRZ32. The pepDA gene consists of a 1,422-bp open reading frame which could encode a polypeptide of 53.5 kDa. No significant identity was found between the deduced amino acid sequence of the pepDA product and the sequence for other polypeptides reported in GenBank. Southern hybridization studies with a pepDA probe indicated that the nucleotide sequence for pepDA is not well conserved among a variety of lactic acid bacteria. Growth studies indicated that a pepDA deletion had no detectable effect on growth rate or acid production by L. helveticus CNRZ32 in milk. Furthermore, no difference in total cellular dipeptidase activity was detected between the mutant and wild-type strains during logarithmic growth in MRS medium.
摘要
此前，从瑞士乳杆菌CNRZ32的基因文库中分离出一种通用二肽酶（pepDA）的基因。pepDA基因由一个1422碱基对的开放阅读框组成，该开放阅读框可编码一个53.5 kDa的多肽。在GenBank中报道的pepDA产物推导氨基酸序列与其他多肽序列之间未发现明显的一致性。用pepDA探针进行的Southern杂交研究表明，pepDA的核苷酸序列在多种乳酸菌中保守性不佳。生长研究表明，pepDA缺失对瑞士乳杆菌CNRZ32在牛奶中的生长速率或产酸没有可检测到的影响。此外，在MRS培养基中对数生长期间，突变株和野生型菌株之间未检测到总细胞二肽酶活性的差异。
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