Colombo E, Magistrelli C, Mendozzi E, Cattaneo E, Achilli G, Ferrante P
Institute of Medical Microbiology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), S. Maria Nascente, University of Milan, Italy.
J Med Virol. 1995 Sep;47(1):10-5. doi: 10.1002/jmv.1890470104.
Six hundred intravenous drug users (IVDUs) and two hundred North Africans were screened for human T-cell leukemia virus (HTLV) antibodies using several serological methods. Eighteen of the eighty-two HTLV-seropositive individuals were also tested by the polymerase chain reaction-DNA enzyme immunoassay (PCR-DEIA), a non-isotopic method of immunoenzymatic detection of the amplified DNA. Of these eighteen subjects, eight IVDUs were found to be HTLV-II-positive by the PCR-DEIA, whereas all of the eighteen subjects were negative for HTLV-I. Western blot (WB) confirmed six of the eight HTLV-positive subjects, while the results of the remaining two were indeterminate. The results confirmed the PCR-DEIA as a rapid and an efficient method of discriminating between HTLV-I and HTLV-II infection, whereas serological tests, including the WB, have limitations in terms of specificity and sensitivity. Moreover, this study showed a higher frequency of HTLV seroreactivity in the Italian IVDU population than in previous studies and confirmed that HTLV-II is more frequent than HTLV-I in this population.
采用多种血清学方法对600名静脉注射吸毒者（IVDU）和200名北非人进行了人类T细胞白血病病毒（HTLV）抗体筛查。对82名HTLV血清学阳性个体中的18人还采用聚合酶链反应 - DNA酶免疫测定法（PCR - DEIA）进行了检测，这是一种检测扩增DNA的非同位素免疫酶法。在这18名受试者中，8名IVDU通过PCR - DEIA检测为HTLV - II阳性，而所有18名受试者的HTLV - I检测均为阴性。蛋白质印迹法（WB）证实了8名HTLV阳性受试者中的6人，其余2人的结果不确定。结果证实PCR - DEIA是一种快速有效的区分HTLV - I和HTLV - II感染的方法，而包括WB在内的血清学检测在特异性和敏感性方面存在局限性。此外，本研究显示意大利IVDU人群中HTLV血清反应性的频率高于以往研究，并证实该人群中HTLV - II比HTLV - I更常见。
