Wattel E, Mariotti M, Agis F, Gordien E, Prou O, Courouce A M, Rouger P, Wain-Hobson S, Chen I S, Lefrère J J
Laboratoire de Biologie Moléculaire, Institut National de Transfusion Sanguine, Paris, France.
J Infect Dis. 1992 Feb;165(2):369-72. doi: 10.1093/infdis/165.2.369.
To confirm the presence of DNA from human T lymphotropic virus type I (HTLV-I), HTLV-II, or both in individuals found HTLV-I/II-positive through systematic screening of blood donations in Guadeloupe (French West Indies), 42 blood donors repeatedly positive for HTLV-I/II by ELISA were studied by polymerase chain reaction (PCR). Three primer pairs (env, pol, tax) targeted on conserved regions of HTLV-I or -II sequences (or both) and six probes (two generic, two HTLV-I-specific, two HTLV-II-specific) were used in a multiplex PCR. HTLV-I sequences were detected in 31 individuals (74%). All 31 subjects positive by Western blot (WB) harbored HTLV-I sequences. Fifteen individuals (48%) were positive with the three primer pairs used, 10 (32%) with two, and 6 (20%) with one. Subjects indeterminate or negative by WB were all negative by PCR. No HTLV-II sequences were detected with specific probes. The results indicate the absence of HTLV-I and -II infection in individuals with indeterminate WB, the presence of HTLV-I DNA in individuals positive for WB in the French West Indies, and the absence of HTLV-II infection in the cohort.
为了确认在瓜德罗普岛（法属西印度群岛）通过系统筛查献血发现的HTLV-I/II阳性个体中是否存在I型人类嗜T淋巴细胞病毒（HTLV-I）、II型人类嗜T淋巴细胞病毒（HTLV-II）或两者的DNA，我们采用聚合酶链反应（PCR）对42名经酶联免疫吸附测定（ELISA）反复检测为HTLV-I/II阳性的献血者进行了研究。在多重PCR中使用了三对靶向HTLV-I或-II序列（或两者）保守区域的引物（env、pol、tax）和六种探针（两种通用探针、两种HTLV-I特异性探针、两种HTLV-II特异性探针）。在31名个体（74%）中检测到了HTLV-I序列。所有31名经蛋白质印迹法（WB）检测为阳性的受试者都携带HTLV-I序列。15名个体（48%）对所使用的三对引物呈阳性，10名（32%）对两对引物呈阳性，6名（20%）对一对引物呈阳性。WB检测结果不确定或为阴性的受试者经PCR检测均为阴性。未用特异性探针检测到HTLV-II序列。结果表明，WB检测结果不确定的个体不存在HTLV-I和-II感染，法属西印度群岛中WB检测为阳性的个体存在HTLV-I DNA，且该队列中不存在HTLV-II感染。
