Mendel I, Clotteau L, Lambert S, Buffet-Janvresse C
Virology Department, Charles Nicolle Hospital, University of Rouen, France.
J Med Virol. 1995 Nov;47(3):231-6. doi: 10.1002/jmv.1890470308.
The prevalence and the characteristics of hepatitis C virus infection (HCV) in 161 HIV-positive patients were studied. HCV seroprevalence was determined by enzyme immunoassay and recombinant immunoblot assay (RIBA). Two different reverse transcriptase polymerase chain reaction (RT-PCR) methods were also used to test the HCV-seropositive samples and 50 EIA-negative sera used as controls. The RNA HCV-positive sera were genotyped by the LiPA procedure. Associations of HCV status with demographic characteristics and risk factors were assessed by chi 2 and Fisher's exact tests. The seroprevalence of HCV was 34.2% with a significant difference between blood and sexual exposure risk groups (60.6% vs. 13.6%, respectively; P < 0.0001). Thirty-six of the 55 anti-HCV-positive sera were also positive for HCV RNA, and PCR detected HCV RNA in 8 HCV-seronegative patients. Various RIBA profiles were found and all sera were positive for antibodies to the c33 protein. A proportion of sera had elevated levels of transaminase activity (37.2%), and abnormal liver function as associated with HCV infection. Forty-two samples were genotyped and five genotypes and subtypes of the HCV virus were detected. Genotype 1a was the most frequent in this cohort, although genotype 1b is generally more common in France. The majority (94.1%) of the patients with genotype 1a had a history of blood exposure, which may account for the difference.
对161例HIV阳性患者的丙型肝炎病毒感染（HCV）患病率及特征进行了研究。通过酶免疫测定和重组免疫印迹测定（RIBA）确定HCV血清阳性率。还使用两种不同的逆转录聚合酶链反应（RT-PCR）方法检测HCV血清阳性样本以及50份用作对照的酶免疫测定阴性血清。通过线性探针分析（LiPA）程序对RNA HCV阳性血清进行基因分型。通过卡方检验和费舍尔精确检验评估HCV状态与人口统计学特征及危险因素之间的关联。HCV血清阳性率为34.2%，血液暴露和性暴露风险组之间存在显著差异（分别为60.6%和13.6%；P<0.0001）。55份抗HCV阳性血清中有36份HCV RNA也呈阳性，并且PCR在8例HCV血清阴性患者中检测到HCV RNA。发现了各种RIBA图谱，所有血清中c33蛋白抗体均呈阳性。一部分血清转氨酶活性水平升高（37.2%），并且肝功能异常与HCV感染相关。对42份样本进行了基因分型，检测到HCV病毒的5种基因型和亚型。1a基因型在该队列中最为常见，尽管1b基因型在法国通常更为常见。1a基因型患者中的大多数（94.1%）有血液暴露史，这可能解释了这种差异。
