肠道病毒70型近期流行毒株的核苷酸序列分析

Nucleotide sequence analysis of recent epidemic strains of enterovirus 70.

作者信息

Yamazaki K, Oishi I, Minekawa Y

机构信息

Laboratory of Virology, Osaka Prefectural Institute of Public Health, Japan.

出版信息

Microbiol Immunol. 1995;39(6):429-32. doi: 10.1111/j.1348-0421.1995.tb02224.x.

DOI:10.1111/j.1348-0421.1995.tb02224.x

PMID:8551976

Abstract

Nucleotide sequences of the genome of enterovirus 70 (EV70) isolated in Osaka in 1993 were determined, and compared with those of the past epidemic strains. Nucleotide substitution rates in 332 bp, 174 bp, and 178 bp of the genes encoding viral protein (VP)1, VP2, or VP3 were 9.0, 7.5, and 5.6% between Kumoi-2/93 and J670/71, respectively. Likewise, the putative amino acid substitution rates were 1.8, 0, and 0%. It seems that the epidemic strains of EV70 in Japan have been evolving at a constant high nucleotide substitution rate but almost all the substitutions were synonymous.

摘要

测定了1993年在大阪分离出的肠道病毒70型（EV70）基因组的核苷酸序列，并与过去的流行毒株进行了比较。在编码病毒蛋白（VP）1、VP2或VP3的基因中，332bp、174bp和178bp的核苷酸替换率在熊野-2/93株和J670/71株之间分别为9.0%、7.5%和5.6%。同样，推测的氨基酸替换率分别为1.8%、0%和0%。看来日本的EV70流行毒株一直在以恒定的高核苷酸替换率进化，但几乎所有的替换都是同义替换。