聚合酶链反应检测急性出血性结膜炎中的肠道病毒70型
Detection of enterovirus 70 by polymerase chain reaction in acute hemorrhagic conjunctivitis.
作者信息
Uchio E, Yamazaki K, Aoki K, Ohno S
机构信息
Department of Ophthalmology, Yokohama City University School of Medicine, Japan.
出版信息
Am J Ophthalmol. 1996 Aug;122(2):273-5. doi: 10.1016/s0002-9394(14)72026-4.
PURPOSE
We have developed and applied reverse transcription-polymerase chain reaction for the detection of enterovirus 70 from conjunctival swabs of patients among the population of an acute hemorrhagic conjunctivitis epidemic on Okinawa, Japan, in 1994.
METHODS
The primers used were selected from the VP1, VP2, and VP3 regions of the enterovirus 70 nucleotide sequence.
RESULTS
Twelve of 27 culture-negative specimens were positive by reverse transcription-polymerase chain reaction.
CONCLUSIONS
The reverse transcription-polymerase chain reaction test provides a reliable diagnostic method that readily allows specific detection of enterovirus 70 in patients with acute hemorrhagic conjunctivitis in whom low enterovirus 70 recovery rates have been obtained recently by cell culture.
目的
我们开发并应用逆转录-聚合酶链反应，从1994年日本冲绳急性出血性结膜炎流行人群中患者的结膜拭子中检测肠道病毒70型。
方法
所用引物选自肠道病毒70型核苷酸序列的VP1、VP2和VP3区域。
结果
27份培养阴性标本中有12份通过逆转录-聚合酶链反应呈阳性。
结论
逆转录-聚合酶链反应试验提供了一种可靠的诊断方法，能够轻松地特异性检测急性出血性结膜炎患者中的肠道病毒70型，而这些患者近期通过细胞培养获得的肠道病毒70型回收率较低。
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