Gaubier P, Wu H J, Laudié M, Delseny M, Grellet F
Laboratoire de Physiologie et Biologie Moléculaire des Plantes, URA 565 du CNRS, Université de Perpignan, France.
Mol Gen Genet. 1995 Nov 1;249(1):58-64. doi: 10.1007/BF00290236.
During the course of an Arabidopsis thaliana genome sequencing project, we identified a gene, G4, with a derived amino acid sequence showing homology to the product of the Rhodobacter capsulatus bchG locus which is involved in the esterification of bacteriochlorophyllide with geranylgeraniol. The relationship between this gene and bchG was confirmed by the isolation and analysis of a corresponding full-length cDNA. Comparison of genomic and cDNA sequences indicated that the gene is made up of 14 exons, some of them being very short. Southern and Northern analyses showed that this sequence represents a single-copy gene and its transcript is detected only in green or greening tissues. Both homologies and expression data suggest that this gene encodes a chlorophyll synthetase, one of the last enzymes of chlorophyll biosynthesis, and thus represents a new example of a nuclear gene encoding an enzyme of this pathway in higher plants.
在拟南芥基因组测序项目过程中，我们鉴定出一个基因G4，其推导的氨基酸序列与红假单胞菌bchG基因座的产物具有同源性，该基因座参与细菌叶绿素酯与香叶基香叶醇的酯化反应。通过分离和分析相应的全长cDNA证实了该基因与bchG之间的关系。基因组和cDNA序列的比较表明，该基因由14个外显子组成，其中一些非常短。Southern和Northern分析表明，该序列代表一个单拷贝基因，其转录本仅在绿色或正在变绿的组织中被检测到。同源性和表达数据均表明，该基因编码叶绿素合成酶，这是叶绿素生物合成的最后几种酶之一，因此代表了高等植物中编码该途径中一种酶的核基因的一个新例子。
