Soll J, Schultz G, Rüdiger W, Benz J
Institut für Tierernährung, Tierärztliche Hochschule Hannover 3000 Hannover, Federal Republic of Germany.
Plant Physiol. 1983 Apr;71(4):849-54. doi: 10.1104/pp.71.4.849.
The reduction of geranylgeranylpyrophosphate to phytylpyrophosphate in spinach chloroplasts is described for the first time. The reductase is localized in the chloroplast envelope. By contrast, the reduction of the geranylgeranyl moiety in Chl synthesis is catalyzed in the thylakoids (via Chl synthetase). NADPH functions as electron donor in both reactions. Chl synthetase is firmly bound to the thylakoid membranes, and very little activity is found in the stroma fraction. Chl synthetase in chloroplasts can use the pyrophosphate ester of either phytol, geranylgeraniol, or farnesol, phytylpyrophosphate being the preferred substrate. Exogenous Chlide exhibits no influence on Chl synthesis by chloroplast subfractions.
首次描述了菠菜叶绿体中香叶基香叶基焦磷酸还原为植基焦磷酸的过程。该还原酶定位于叶绿体被膜。相比之下，叶绿素合成中香叶基香叶醇部分的还原是在类囊体中由叶绿素合成酶催化的。在这两个反应中，NADPH作为电子供体。叶绿素合成酶与类囊体膜紧密结合，在基质部分几乎没有活性。叶绿体中的叶绿素合成酶可以使用叶绿醇、香叶基香叶醇或法呢醇的焦磷酸酯，植基焦磷酸是首选底物。外源叶绿素酸酯对叶绿体亚组分的叶绿素合成没有影响。
