Rzymowska J, Skierski J, Kurylcio L, Dyrda Z
Department of Cell Biology, Maria Sklodowska-Curie University, Lublin, Poland.
Neoplasma. 1995;42(5):239-42.
Enzymatically dissociated cells exhibited growth in most cases while mechanically dissociated cells did not grow in short term cultures and were not suitable for cytometric study. Breast cancer cells obtained by enzymatic dissociation were examined for DNA content and S-phase fraction. The most of the primary and metastatic breast cancer cells were aneuploid. The 74% rate of invasive and 62% of primary breast cancers had aneuploid (> G0/G1) DNA content, 22% of metastatic breast cancer cells and 37% of primary tumors cells were DNA diploid or near diploid tumor stemlines. The means of DNA diploid and near diploid tumor cells were higher in primary tumors. DNA index was higher in more aggressive and metastatic tumors. S-phase fractions were higher in cells of metastatic tumors than those in primary tumors. Nuclear DNA content and S-phase fraction can be considered a valuable prognostic indicators in breast cancer.
大多数情况下，酶解细胞能够生长，而机械解离的细胞在短期培养中无法生长，不适合进行细胞计数研究。对通过酶解获得的乳腺癌细胞进行了DNA含量和S期比例检测。大多数原发性和转移性乳腺癌细胞为非整倍体。74%的浸润性乳腺癌和62%的原发性乳腺癌具有非整倍体（> G0/G1）DNA含量，22%的转移性乳腺癌细胞和37%的原发性肿瘤细胞为DNA二倍体或近二倍体肿瘤干细胞系。原发性肿瘤中DNA二倍体和近二倍体肿瘤细胞的平均值更高。在侵袭性更强和转移性肿瘤中，DNA指数更高。转移性肿瘤细胞的S期比例高于原发性肿瘤细胞。核DNA含量和S期比例可被视为乳腺癌中有价值的预后指标。
