Araya J C, Roa I, Wistuba I, Villaseca M A, Contreras E, Olcese A, Danton A, Watanabe H
Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
Rev Med Chil. 1994 Jun;122(6):643-52.
There is evidence that DNA quantization and histopathological classification of breast cancer may be useful for its therapeutic management. DNA flow cytometry, clinical and anatomopathological features of 60 paraffin embedded primary breast cancer tissue samples were studied. The aneuploidy percentage was 67%. There was a correlation between DNA index and degree of cellular pleomorphism, degree of differentiation and the fraction of cells in S phase. Likewise a correlation was found between the degree of cellular pleomorphism and the mitotic index. DNA cytophotometry was useful to solve cases of difficult diagnosis with flow cytometry ("near" diploid and tetraploid tumors). No correlation was found between aneuploidy, percentage of cells in phase S, degree of cellular atypia and mitotic degree with clinical stage, degree of lymph node involvement, tumoral size or age. It is suggested that these variables may have an independent behavior.
有证据表明，乳腺癌的DNA量化和组织病理学分类可能有助于其治疗管理。对60份石蜡包埋的原发性乳腺癌组织样本的DNA流式细胞术、临床和解剖病理学特征进行了研究。非整倍体百分比为67%。DNA指数与细胞多形性程度、分化程度和S期细胞分数之间存在相关性。同样，细胞多形性程度与有丝分裂指数之间也存在相关性。DNA细胞光度术有助于解决流式细胞术诊断困难的病例（“近”二倍体和四倍体肿瘤）。未发现非整倍体、S期细胞百分比、细胞异型性程度和有丝分裂程度与临床分期、淋巴结受累程度、肿瘤大小或年龄之间存在相关性。建议这些变量可能具有独立的行为。
