Greiner J V, Leahy C D, Glonek T
Schepens Eye Research Institute, Boston, MA 02114, USA.
Ophthalmic Res. 1995;27(5):277-85. doi: 10.1159/000267737.
Since the uveal tract becomes involved in inflammatory disorders, which are known to affect lipid metabolism, we studied normal membrane phospholipids (PLs) in order to (1) determine baseline PL profiles of the iris, ciliary body, and choroid, and (2) compare and contrast PL profiles of the uveal tissues.
Iris, ciliary body and choroid tissues were isolated from rabbit eyes (n = 30) and extracted with chloroform-methanol using a modified Folch procedure. Quantitative tissue PL profiles were obtained using 31P nuclear magnetic resonance.
Fourteen PLs were detected and quantitated in all three uveal tissues among which was one unidentified PL at -0.17 ppm. The five major PLs in the iris, ciliary body and choroid, respectively, have the following PL composition (mole percent of total phosphorus): ethanolamine plasmalogen 14.58, 15.07, 16.52; phosphatidylethanolamine 13.10, 12.40, 9.23; phosphatidylserine 11.24, 10.27, 12.13; sphingomyelin (SM) 11.10, 11.97, 18.21; and phosphatidylcholine (PC) 36.61, 36.70, 29.88. Additionally, lysophosphatidic acid, phosphatidic acid, lysophosphatidylcholine, phosphatidylinositol, and PC plasmalogen or alkacyl PC were detected in all tissues. Sphingosylphosphorylcholine also was detected in the ciliary body and choroid. Lysophosphatidylethanolamine was detected in the choroid. In addition, 42 PL metabolic indexes were calculated from these data, which permitted pathway-specific lipid analyses.
This study establishes baseline PL profiles of the uveal tract tissues and will permit comparisons with tissues from eyes with inflammatory disorders. The PL concentrations in conjunction with the indexes demonstrate that overall the choroid has membranes that are less permeable to ion translocation than either the iris or the ciliary body, although there are compensatory concentration changes between the SM and PC components among these three tissues.
由于葡萄膜会参与炎症性疾病，而炎症性疾病已知会影响脂质代谢，因此我们研究了正常的膜磷脂（PLs），以便（1）确定虹膜、睫状体和脉络膜的基线PL谱，以及（2）比较和对比葡萄膜组织的PL谱。
从兔眼（n = 30）中分离出虹膜、睫状体和脉络膜组织，并使用改良的Folch法用氯仿 - 甲醇进行提取。使用31P核磁共振获得定量的组织PL谱。
在所有三种葡萄膜组织中检测并定量了14种PL，其中在-0.17 ppm处有一种未鉴定的PL。虹膜、睫状体和脉络膜中五种主要的PL分别具有以下PL组成（占总磷的摩尔百分比）：乙醇胺缩醛磷脂14.58、15.07、16.52；磷脂酰乙醇胺13.10、12.40、9.23；磷脂酰丝氨酸11.24、10.27、12.13；鞘磷脂（SM）11.10、11.97、18.21；以及磷脂酰胆碱（PC）36.61、36.70、29.88。此外，在所有组织中均检测到溶血磷脂酸、磷脂酸、溶血磷脂酰胆碱、磷脂酰肌醇以及PC缩醛磷脂或烷酰基PC。在睫状体和脉络膜中也检测到鞘氨醇磷酸胆碱。在脉络膜中检测到溶血磷脂酰乙醇胺。此外，根据这些数据计算了42种PL代谢指标，这允许进行特定途径的脂质分析。
本研究建立了葡萄膜组织的基线PL谱，并将允许与患有炎症性疾病的眼睛的组织进行比较。PL浓度与指标表明，总体而言，脉络膜的膜对离子转运的通透性低于虹膜或睫状体，尽管这三种组织中SM和PC成分之间存在补偿性浓度变化。
