Coughlan S N, Saman E, Jacobs D, Mercier C, Cesbron-Delauw M F, Trees A J
Department of Veterinary Parasitology, Liverpool School of Tropical Medicine, UK.
Parasite Immunol. 1995 Sep;17(9):465-8. doi: 10.1111/j.1365-3024.1995.tb00916.x.
Immune responses to recombinant fragments of the Toxoplasma gondii antigens ROP2 and GRA2 were investigated in sheep naturally and experimentally infected with T. gondii oocysts. Specific serum antibodies to C-terminal fragments were detected by ELISA. Cell-mediated responses in peripheral blood mononuclear cells were demonstrated by proliferation and interferon-gamma production following in vitro stimulation with the ROP2 fragment. This data indicates the presence of epitopes for sheep B cells in the recombinant GRA2 fragment and for both B and T cells in the ROP2 fragment.
在自然感染和实验感染刚地弓形虫卵囊的绵羊中，研究了对刚地弓形虫抗原ROP2和GRA2重组片段的免疫反应。通过ELISA检测针对C端片段的特异性血清抗体。用ROP2片段进行体外刺激后，通过增殖和干扰素-γ产生证明外周血单核细胞中的细胞介导反应。该数据表明重组GRA2片段中存在绵羊B细胞的表位，ROP2片段中存在B细胞和T细胞的表位。
