基于重组抗原的酶联免疫吸附测定法的开发，用于检测绵羊和猫体内的弓形虫特异性抗体。
Development of ELISAs based on recombinant antigens for the detection of Toxoplasma gondii-specific antibodies in sheep and cats.
作者信息
Tenter A M, Vietmeyer C, Johnson A M
机构信息
Institut für Parasitologie, Tierärztliche Hochschule Hannover, Germany.
出版信息
Vet Parasitol. 1992 Jul;43(3-4):189-201. doi: 10.1016/0304-4017(92)90160-b.
ELISAs using recombinant parasite polypeptides as antigens were developed to measure Toxoplasma gondii-specific antibodies in the sera of sheep and cats. Compared with an ELISA based on traditional parasite antigen, the ELISA for sheep sera had a sensitivity of 79% and a negative predictive value of 80%, and the ELISA for cat sera had a sensitivity of 100% and a negative predictive value of 100%. Both ELISAs had specificities of 100% and positive predictive values of 100%. These ELISAs appear to be a useful cost-effective alternative to ELISAs based on traditional parasite antigen for the measurement of T. gondii-specific antibodies in the sera of sheep and cats.
开发了以重组寄生虫多肽作为抗原的酶联免疫吸附测定（ELISA），用于检测绵羊和猫血清中的弓形虫特异性抗体。与基于传统寄生虫抗原的ELISA相比，用于绵羊血清的ELISA灵敏度为79%，阴性预测值为80%，用于猫血清的ELISA灵敏度为100%，阴性预测值为100%。两种ELISA的特异性均为100%，阳性预测值均为100%。对于检测绵羊和猫血清中的弓形虫特异性抗体而言，这些ELISA似乎是基于传统寄生虫抗原的ELISA的一种有用且具成本效益的替代方法。
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