Menéndez R, Marco V, Rubio V, Solé A
Servicio de Neumología, Hospital Universitario La Fe, Valencia, España.
Respiration. 1995;62(6):312-6. doi: 10.1159/000196472.
Fibronectin (FN) is a glycoprotein present in the epithelial surface of airways and in the interstitial parenchyma of the lung. The aim of this study was to quantify the amount of FN in human lung parenchyma, and to investigate possible differences between smokers and non-smokers, both in lung parenchyma and in bronchoalveolar lavage (BAL) fluid. BAL fluid was recovered in 21 individuals. Quantification of FN in BAL was done by a non-competitive ELISA. Twenty-six lung parenchyma samples were also obtained from lung resections. FN was extracted with urea and heparin. Quantification of FN in lung parenchyma was determined by a competitive ELISA. The mean +/- SD FN content in BAL fluid from smokers was 1.59 +/- 1.65 micrograms/mg total protein and 0.53 +/- 0.26 in non-smokers (p < 0.05). However, the amount of FN/ml BAL fluid was not statistically different between smokers (0.15 +/- 0.13 micrograms/ml) and non-smokers (0.106 +/- 0.07). The mean extractable FN in human lung tissue was 38.6 micrograms/g wet weight (31.7-45.5, 95% mean confidence interval) with no statistical difference between smokers and non-smokers. We conclude that in smokers, FN accounts for a higher proportion of the total protein in BAL fluid, and that there were no differences in FN content in lung parenchyma between smokers and non-smokers.
纤连蛋白（FN）是一种存在于气道上皮表面和肺间质实质中的糖蛋白。本研究的目的是量化人肺实质中FN的含量，并调查吸烟者和非吸烟者在肺实质和支气管肺泡灌洗（BAL）液中的可能差异。对21名个体采集了BAL液。通过非竞争性酶联免疫吸附测定（ELISA）对BAL中的FN进行定量。还从肺切除术中获取了26个肺实质样本。用尿素和肝素提取FN。通过竞争性ELISA测定肺实质中FN的含量。吸烟者BAL液中FN的平均含量±标准差为1.59±1.65微克/毫克总蛋白，非吸烟者为0.53±0.26（p<0.05）。然而，吸烟者（0.15±0.13微克/毫升）和非吸烟者（0.106±0.07）之间每毫升BAL液中FN的含量无统计学差异。人肺组织中可提取的FN平均含量为38.6微克/克湿重（31.7 - 45.5，95%平均置信区间），吸烟者和非吸烟者之间无统计学差异。我们得出结论，在吸烟者中，FN在BAL液总蛋白中所占比例更高，并且吸烟者和非吸烟者肺实质中FN含量无差异。
